sp6 promoter primer (Danaher Inc)
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Sp6 Promoter Primer, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sp6+promoter+primer/SP6+Promoter/pmc09207569-56-0-4
Average 94 stars, based on 31 article reviews
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1) Product Images from "In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly"
Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
Journal: STAR Protocols
doi: 10.1016/j.xpro.2022.101458
Figure Legend Snippet: Features and use of a synthesized vector using the presented protocol for riboprobe and inhibitory RNA synthesis for planaria Smed-Slit-1 (A) The constructed vector can be used for both the synthesis of sense (with T3) and antisense (with SP6) riboprobes as well as dsRNA (with T7) for RNAi. (B) Sense riboprobes of Smed-Slit-1 produce no staining. (C) Antisense riboprobes of Smed-Slit-1 produce a characteristic midline expression pattern (arrows). (D) Head amputation in worms fed control dsRNA for GFP results in the regeneration of two eyes (red arrows). (E) Head amputation in worms fed dsRNA for Smed-Slit-1 results in the regeneration of a single eye at the midline (red arrow). Scale bars, 100 μm.
Techniques Used: Synthesized, Plasmid Preparation, Construct, Staining, Expressing, Control
Figure Legend Snippet:
Techniques Used: Virus, Recombinant, Labeling, Purification, Plasmid Preparation, cDNA Synthesis, Software
Figure Legend Snippet:
Techniques Used: Plasmid Preparation
Figure Legend Snippet: Riboprobe Synthesis Reaction
Techniques Used: Labeling
Related Articles
Synthesized:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. Plasmid Preparation:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. Construct:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. Staining:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. Expressing:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. Control:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. Virus:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. Recombinant:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. Labeling:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. Purification:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. cDNA Synthesis:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. Software:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. In Situ Hybridization:Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. |