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sp6 promoter primer  (Danaher Inc)


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    Structured Review

    Danaher Inc sp6 promoter primer
    Features and use of a synthesized vector using the presented protocol for riboprobe and inhibitory RNA synthesis for planaria Smed-Slit-1 (A) The constructed vector can be used for both the synthesis of sense (with T3) and antisense (with <t>SP6)</t> riboprobes as well as dsRNA (with T7) for RNAi. (B) Sense riboprobes of Smed-Slit-1 produce no staining. (C) Antisense riboprobes of Smed-Slit-1 produce a characteristic midline expression pattern (arrows). (D) Head amputation in worms fed control dsRNA for GFP results in the regeneration of two eyes (red arrows). (E) Head amputation in worms fed dsRNA for Smed-Slit-1 results in the regeneration of a single eye at the midline (red arrow). Scale bars, 100 μm.
    Sp6 Promoter Primer, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sp6+promoter+primer/SP6+Promoter/pmc09207569-56-0-4
    Average 94 stars, based on 31 article reviews
    sp6 promoter primer - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly"

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2022.101458

    Features and use of a synthesized vector using the presented protocol for riboprobe and inhibitory RNA synthesis for planaria Smed-Slit-1 (A) The constructed vector can be used for both the synthesis of sense (with T3) and antisense (with SP6) riboprobes as well as dsRNA (with T7) for RNAi. (B) Sense riboprobes of Smed-Slit-1 produce no staining. (C) Antisense riboprobes of Smed-Slit-1 produce a characteristic midline expression pattern (arrows). (D) Head amputation in worms fed control dsRNA for GFP results in the regeneration of two eyes (red arrows). (E) Head amputation in worms fed dsRNA for Smed-Slit-1 results in the regeneration of a single eye at the midline (red arrow). Scale bars, 100 μm.
    Figure Legend Snippet: Features and use of a synthesized vector using the presented protocol for riboprobe and inhibitory RNA synthesis for planaria Smed-Slit-1 (A) The constructed vector can be used for both the synthesis of sense (with T3) and antisense (with SP6) riboprobes as well as dsRNA (with T7) for RNAi. (B) Sense riboprobes of Smed-Slit-1 produce no staining. (C) Antisense riboprobes of Smed-Slit-1 produce a characteristic midline expression pattern (arrows). (D) Head amputation in worms fed control dsRNA for GFP results in the regeneration of two eyes (red arrows). (E) Head amputation in worms fed dsRNA for Smed-Slit-1 results in the regeneration of a single eye at the midline (red arrow). Scale bars, 100 μm.

    Techniques Used: Synthesized, Plasmid Preparation, Construct, Staining, Expressing, Control


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Labeling, Purification, Plasmid Preparation, cDNA Synthesis, Software


    Figure Legend Snippet:

    Techniques Used: Plasmid Preparation

    Riboprobe Synthesis Reaction
    Figure Legend Snippet: Riboprobe Synthesis Reaction

    Techniques Used: Labeling

    Related Articles

    Synthesized:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    Plasmid Preparation:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    Construct:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    Staining:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    Expressing:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    Control:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    Virus:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    Recombinant:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    Labeling:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    Purification:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    cDNA Synthesis:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    Software:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.

    In Situ Hybridization:

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
    Article Snippet: T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.T3 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-03.. SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.. T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.T7 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-20-01.



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    Features and use of a synthesized vector using the presented protocol for riboprobe and inhibitory RNA synthesis for planaria Smed-Slit-1 (A) The constructed vector can be used for both the synthesis of sense (with T3) and antisense (with <t>SP6)</t> riboprobes as well as dsRNA (with T7) for RNAi. (B) Sense riboprobes of Smed-Slit-1 produce no staining. (C) Antisense riboprobes of Smed-Slit-1 produce a characteristic midline expression pattern (arrows). (D) Head amputation in worms fed control dsRNA for GFP results in the regeneration of two eyes (red arrows). (E) Head amputation in worms fed dsRNA for Smed-Slit-1 results in the regeneration of a single eye at the midline (red arrow). Scale bars, 100 μm.
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    Features and use of a synthesized vector using the presented protocol for riboprobe and inhibitory RNA synthesis for planaria Smed-Slit-1 (A) The constructed vector can be used for both the synthesis of sense (with T3) and antisense (with <t>SP6)</t> riboprobes as well as dsRNA (with T7) for RNAi. (B) Sense riboprobes of Smed-Slit-1 produce no staining. (C) Antisense riboprobes of Smed-Slit-1 produce a characteristic midline expression pattern (arrows). (D) Head amputation in worms fed control dsRNA for GFP results in the regeneration of two eyes (red arrows). (E) Head amputation in worms fed dsRNA for Smed-Slit-1 results in the regeneration of a single eye at the midline (red arrow). Scale bars, 100 μm.
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    Image Search Results


    Features and use of a synthesized vector using the presented protocol for riboprobe and inhibitory RNA synthesis for planaria Smed-Slit-1 (A) The constructed vector can be used for both the synthesis of sense (with T3) and antisense (with SP6) riboprobes as well as dsRNA (with T7) for RNAi. (B) Sense riboprobes of Smed-Slit-1 produce no staining. (C) Antisense riboprobes of Smed-Slit-1 produce a characteristic midline expression pattern (arrows). (D) Head amputation in worms fed control dsRNA for GFP results in the regeneration of two eyes (red arrows). (E) Head amputation in worms fed dsRNA for Smed-Slit-1 results in the regeneration of a single eye at the midline (red arrow). Scale bars, 100 μm.

    Journal: STAR Protocols

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly

    doi: 10.1016/j.xpro.2022.101458

    Figure Lengend Snippet: Features and use of a synthesized vector using the presented protocol for riboprobe and inhibitory RNA synthesis for planaria Smed-Slit-1 (A) The constructed vector can be used for both the synthesis of sense (with T3) and antisense (with SP6) riboprobes as well as dsRNA (with T7) for RNAi. (B) Sense riboprobes of Smed-Slit-1 produce no staining. (C) Antisense riboprobes of Smed-Slit-1 produce a characteristic midline expression pattern (arrows). (D) Head amputation in worms fed control dsRNA for GFP results in the regeneration of two eyes (red arrows). (E) Head amputation in worms fed dsRNA for Smed-Slit-1 results in the regeneration of a single eye at the midline (red arrow). Scale bars, 100 μm.

    Article Snippet: SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.

    Techniques: Synthesized, Plasmid Preparation, Construct, Staining, Expressing, Control

    Journal: STAR Protocols

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly

    doi: 10.1016/j.xpro.2022.101458

    Figure Lengend Snippet:

    Article Snippet: SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.

    Techniques: Virus, Recombinant, Labeling, Purification, Plasmid Preparation, cDNA Synthesis, Software

    Journal: STAR Protocols

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly

    doi: 10.1016/j.xpro.2022.101458

    Figure Lengend Snippet:

    Article Snippet: SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.

    Techniques: Plasmid Preparation

    Riboprobe Synthesis Reaction

    Journal: STAR Protocols

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly

    doi: 10.1016/j.xpro.2022.101458

    Figure Lengend Snippet: Riboprobe Synthesis Reaction

    Article Snippet: SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.

    Techniques: Labeling